DNA

Part:BBa_K3942002:Experience

Designed by: Igor Blatov   Group: iGEM21_MIPT_MSU   (2021-10-12)


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Applications of BBa_K3942002

We fused this part with GFP and lacZ, so after translation it will be selected by the gag-polyprotein and via ESCRT pathway packaged into vesicles. However, after collecting vesicles and adding them to the targeted cell line, HT-29, we did not observe GFP luminescence within first 24 hours.

Overall, it is still could be used as a packaging signal, but due to increased length of translation needed for this construction(when compared to psi short), it might take longer time for vesicles to accumulated, and for them to have effect within target.


User Reviews

UNIQ2180cd38fc10aecc-partinfo-00000000-QINU UNIQ2180cd38fc10aecc-partinfo-00000001-QINU